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thereafter, 50 ml of precultures were induced with 450 ml of expression medium (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 bacto peptone (Gibco, 211677), 20 g l 1 d-galactose (Panreac, A1131), 67 mM potassium phosphate monobasic buffer pH 6.0 (Thermo Scientific, 424200250), 25 g l 1 ethanol (Scharlau, ET00062500), 2 mM copper sulfate (Merck, C7631) and 25 g ml 1 chloramphenicol (Merck, C0378)) in a 2.5-l baffled flask (Quimigen, 931136-B) (OD 600 = 0.1)

Do not apply to broken skin

The Nrf2 regulatory network provides an interface between redox and intermediary metabolism

Always check with your knowledgeable herp vet before administering any antibiotic

Adoptive transfer As described 52 , splenocytes were collected by gently pushing spleens through a 100-m strainer, and red blood cells were lysed with ACK lysing buffer (Gibco, A10492-01)