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KG), SDS was then added and the cells were mixed with the color reagent (TBA in acetic acid and sodium hydroxide) in a boiling water bath for 1 h, cooled in an ice-water bath, and then incubated on ice

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We also confirmed that the siPOOL siRNA against ALK5 did not lead to changes in the mRNA expression of ALK4 or ALK7 in both untransduced cells and aSyn-overexpressing cells (Fig