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Finally, the gel was detained in 100 mL of destain solution (containing 8% acetic acid) by heating in the microwave (9501100 watts) for 45 seconds and shaking on an orbital shaker at room temperature until the desired background was achieved

In few instances the crude cell suspension is layered on a discontinuous gradient of 8.2% and 15.6 % (w/v) Nycodenz [14,15] but this gradient format is normally reserved for the purification of stellate cells and Kupffer cells