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For each filament, the number of applications remains constant (510 applications in general), and the number of positive responses (out of those 510 applications) is converted into percent response. The median 50% PWT is then calculated (Chaplan et al., 1994)

Longer active phases suit focused protocols targeting specific outcomes like post-surgical healing or seasonal anti-aging pushes

Blood samples were obtained from the tail vein and glucose measurements were performed in duplicate using Accu-Chek Active glucometers

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Wasade) 118 (Cambridge University Press, 2019)