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Abstract The growth factor and small molecule protocol are the two primary approaches for generating human induced pluripotent stem cell-derived hepatocyte-like cells (iPSC-HLCs)

Spinning disk confocal microscopy For clear organoid visualization, we used the ultrafast confocal system IXplore SpinSR Olympus (Olympus, Tokyo, Japan)

NAD+ contributes to several important functions including: repairing failing mitochondria (the powerhouse of our cells), DNA repair, and providing fuel for some important immune modulators

It is naturally synthesized by every cell in the body, with particularly elevated concentrations found in the liver

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