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Fixed tumours were analysed by epifluorescence for in situ apoptosis with terminal-deoxynucleotidyl-transferase-mediated dUTP nick-end labelling (TUNEL) (Roche) or for in situ NF- B activation with rabbit anti-P65 Abs revealed with fluorescein-labelled goat anti-rabbit Abs, and nuclei were stained with DAPI

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