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10.1158/0008-5472.CAN-07-2489 63 GalluzziL.MorselliE.KeppO.VitaleI.RigoniA.VacchelliE.et al (2010)

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A plant-based mutant huntingtin model-driven discovery of impaired expression of GTPCH and DHFR

Beads were repeatedly washed with GST-lysis buffer, prepared for SDS-PAGE, and finally analyzed by Western blot using antibodies against GFP, mRFP, and Flag-tag when needed, as well as HRP-conjugated secondary antibodies for ECL detection