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The signal peptide prediction tool named SPScan from GCG package also demonstrates an obvious cleavage site between residues 24 and 25 ( Brain and testis poly A+RNA was purchased from Clontech and converted into double stranded cDNA using the SuperScript Plasmid System for cDNA Synthesis and Plasmid Cloning (Life Technologies) except that no radioisotope was incorporated in either of the cDNA synthesis steps and that the cDNA was fractionated by HPLC

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