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Applied to DBSs on cellulose or cotton filter paper, the method effectively quantified carnitines and assessed their stability under two conditions: a single 4-hour sunlight exposure and cyclic temperature changes (-20 C for 2 days, 40 C for 2 days, and back to -20 C for 2 more days)

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Recent investigations have confirmed that intestinal flora disorders can cause metabolic ailments and inflammatory reactions through metabolism and the immune system, leading to the formation and rupture of atherosclerotic plaques (8, 9)

Additionally, RTT patients have an increased incidence of unexpected death, and often die due to respiratory infection, cardiac instability, and respiratory failure (Laurvick et al