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This renders the cells non-viable

For this purpose, CD4 + T cells were left untreated or treated for 48 h with AF, BSO, or a combination of the two, and cells were then sorted for viability (Appendix Fig S8A) to eliminate the potential bias of the general cytotoxic effect of AF on lymphocytes (Chirullo et al , 2013) (Appendix Fig S8B)