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Pawankar et al., 2011

N-acetyl cysteine (NAC) is a supplement derived from cysteine, a sulfur-containing amino acid that is crucial for the synthesis of glutathione

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The high sensitivity (100% for the known carcinogenic and mutagenic NAs tested here) of HepG2 cells supplemented with hamster S9-mix, as compared to PHH and PRH, in principle might make this system a valuable tool for hazard identification and potential follow up of negative/borderline EAT test results to provide further reassurance for the EAT result