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The cell line was seeded in a 6-well plate at a concentration of 4105 cells/2 ml and cultured in Dulbecco's modified Eagle's medium (DMEM) suppl emented with 10% fetal calf serum (FCS) (both from Gibco Life Technologies, Grand Island, NY, USA) and 1% penicillin-streptomycin at 37C in a 5% CO2 atmosphere

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