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We thus repeated the hypoxia-reoxygenation experiments in leaves with NAPstar4.3, NAPstarC, Peredox, roGFP2-Grx1 and NERNST sensors ( Figure S10 ) The NERNST response was almost indistinguishable from a roGFP2-Grx1 response ( Figure S10C,D ) consistent with NERNST responding predominantly to changes in E GSH in the Arabidopsis cytosol