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Cell pellets were resuspended in lysis buffer (250 mM HEPES, 2% SDS, pH 7.0), and N-ethylmaleimide(NEM) was added to the lysis buffer to block the free thiols (the final concentration was 100 mM), The samples were then sonicated 12 times for 10 s each time followed by intervals of 30 s on ice for cooling

"Three-Dimensional Brain Metabolic Imaging in Patients with Toxic Encephalopathy

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They frequently clustered together, occupying a large volume within the cytosol that were not enclosed by lipid membranes

The autosampler (SIL-20AC HT) and the column temperature were maintained at 4 C and 25 C, respectively