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After washing the membranes with PBS-T three times for 5 min each, they were incubated with HRP-conjugated secondary antibodies [anti-rabbit IgG, anti-goat IgG or anti-mouse IgG (1:2000, Santa Cruz, CA, USA)] for 1 h with rocking and then washed with PBS-T 3times for 20 min

Res 130 , 418431 (2022)

Upregulation of macrophage migration inhibitory factor (MIF) and CD74, receptor for MIF, in rat bladder during persistent cyclophosphamide-induced inflammation

Studies suggest oral bioavailability ranges from 3-30% depending on formulation, with liposomal preparations showing improved absorption compared to standard capsules