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Real-time quantitative PCR analysis Using the RNAiso Plus kit, total RNA was extracted from gill tissue samples, and cDNA was produced via reverse transcription with the Prime Script RT kit

These stimuli promote the activation of M1 microglial cells, triggering neuroinflammation and leading to the loss of dopaminergic neurons [138]

These studies on myocardial injury and the GSH system reveal new roles of these clinical drugs in the treatment and further elaborate their mechanisms, which provide new ideas for their clinical application

For quantitative analysis, GCC was measured by circular-scanning around the optic nerve disc (Fig

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