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Mobile phase A consisted of 0.01% NH4OH in water and mobile phase B of 0.01% NH4OH in methanol with a gradient starting at 5% mobile phase B which linearly increased to 90% B at 2 min and to 100% B at 2.5 min

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Van Remmen, H., Ikeno, Y., Hamilton, M., Pahlavani, M., Wolf, N., Thorpe, S

Considering the high plasticity of microglia according to different pathologic events or microenvironments [33], deeper insight into the regulatory mechanisms of microglial phenotype variations may help maintain the homeostasis of microglia activation