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Protein concentrations were determined using an enhanced BCA protein assay kit (Beyotime)

Although side effects derived from spearmint and peppermint are often suggested which includes allergy and iron deficiency anemia [104], the free testosterone and triglyceride were decreased, while LH, FSH and estradiol levels were increased in Menta herbal tea-drunk PCOS hirsutism women [104]

We also examine how processing-related stressors influence bacterial physiology and resistance traits, contributing to a more mechanistic understanding of pathogen adaptation in food-associated environments

Fluorescent microscopy analysis GFP expression and quantification were carried out as described previously 6