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The lipid mixture was either stored at 80 C or immediately extruded using an Avanti Polar Lipids Mini Extruder (610023) at least 41 times through a 200-nm filter (Whatman Nuclepore Track-Etched Membranes, diameter of 19 mm) for mTORC1 kinase activity assays and cryo-EM studies

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The results demonstrated that MT2 administration improved social behavioral metrics, which had been impaired in the MIA mice

The proportion achieving clinically significant weight loss also increased with dose