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Colchicine for the secondary prevention of cardiovascular events

Sterile water has no preservative

The lysates were centrifuged at 4 C for 5 min, and supernatants were incubated with anti-SIRT1 (Ab110304, Abcam) or anti-SIRT3 (Ab246522, Abcam) antibodies for 4 h at 4 C