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Loss of the tumor suppressor LKB1 promotes metabolic reprogramming of cancer cells via HIF-1

After primary antibody incubation, slices were then washed with PBS three times and incubated with secondary antibody donkey anti-mouse IgG (H + L) Alexa Fluor 488 (1:1000, Thermofisher Scientific, A-21202) in blocking solution for 90 min