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Mass spectrometry of the corresponding SDS gel area detected GspD (Table S2), indicating that these cross-reacting bands represent a protein that underwent proteolytic degradation during sample handling - a phenomenon often observed in M

Primary antibodies for western blotting Immunoblotting was performed with rabbit polyclonal anti-GCL (1:1,000) 5 , rabbit polyclonal anti-GPX1 (1:1,000) (ab59546, Abcam, Cambridge, UK), rabbit polyclonal anti-GFP (1:1,000) (ab290, Abcam), mouse monoclonal anti-GSS (1:1,000) (Sc-166882, Santa Cruz Biotechnologies, Heidelberg, Germany), mouse monoclonal anti-GSR (Sc-133159, Santa Cruz Biotechnologies), mouse monoclonal anti-SOD2 (1:2,000) (ab16,956, Abcam), rabbit polyclonal anti-VDAC (1:1,000) (Calbiochem, Darmstadt, Germany) or mouse monoclonal anti-GAPDH (1:40,000) (glyceraldehyde-3-phosphate dehydrogenase

GraphPad, San Diego, CA, U.S.A)