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Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml ) antibodies, in RPMI medium or DMEM, supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1), in the presence of IL-2 (10 ng ml 1 , Peprotech) and IL-12 (10 ng ml 1 , Peprotech) (T H 1 conditions), with or without amino acids

Current human surveillance does not show increased MTC incidence with GLP-1 use, but informed decision-making and closer thyroid ultrasound screening may be warranted in high-risk individuals

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J.CushmanM.et al (2017)

doi: 10.1016/j.plantsci.2013.01.005 90 GrunewaldW.De SmetI.LewisD