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Transmission electron microscopy M0 macrophages were stimulated in 6-well plates (5 10 5 cells per well) as described above and then fixed for 30 min with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (Thermo Fisher Scientific)

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When They Should Not Be First-Line Therapy Despite their efficacy, novel weight loss peptides should generally not replace GLP-1 or GIP receptor agonists as initial therapy for uncomplicated obesity

Ribosome biogenesis acts downstream of Trp prototrophy and inhibits Gcn4 induction Three parallel lines of evidence converged on the hypothesis that the ribosome biogenesis pathway mediates the repressive effect on Gcn4 induction by Trp metabolism: First, under nutrient-rich conditions, ribosomal proteins can repress the translation of GCN4 mRNA as a regulatory mechanism to maintain the Gcn4 protein at a relatively low basal level 51