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Lentiviral transfection and establishment of stable cell lines To knock down specific target genes, such as human GPX4 (NM_001039847.3), Raji cells were infected with a lentivirus encoding GPX4 or a control virus

5,514 Sirt1, a protein deacetylase, plays a key role in delaying cellular aging and regulating metabolism

The two sequential ATP-dependent reactions by which glutathione is synthesized inside cells, with the enzyme catalyzing the reaction and the number of ATP molecules required for the reaction to occur, displayed above and below the arrows respectively

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