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Samples were centrifuged at 16,000 g for 10 min at 4 C, and 200 l of supernatant was mixed with 200 l of 2,4-dinitrophenyl-hydrazine solution (0.05 M in acetonitrile:acetic acid 9:1 (v/v))

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Acknowledgments The authors acknowledge kind sponsorship by Ferring Pharmaceuticals to support in vitro experimental work to determine the role of GH in carbohydrate and lipid metabolism in granulosa cell lines performed in the laboratory of KK and JY

Exosomes communicate protective messages during oxidative stress