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These tissue sections were deparaffinized in graded xylene, rehydrated in graded alcohol solutions, and then incubated in antigen retrieval buffer (Roche, Basel, Switzerland) at 37 C for 30 min

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The distinction matters enormously: it is the difference between exposing cells to a free synthetic peptide of uncertain stability and having living tissue produce the E-domain in its natural molecular and cellular context

Asparagus is full of antioxidants like vitamins C and E, glutathione, and rutin