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The proteins were neutralized and solvated in 0.15 M NaCl solution using the simple point-charge water model and a box size of 7.0 7.0 7.0 nm 3

For H 2 O 2 assessment in isolated mitochondria, 50 g protein-aliquots of freshly obtained mitochondria were incubated with 100 M AmplexRed probe (Invitrogen) in mitochondrial respiration buffer (125 mM KCl, 2 mM KH 2 PO 4 , 1 mM MgCl 2 , 0.5 mg ml 1 bovine serum albumin and 10 mM HEPES, pH 7.4) containing horseradish peroxidase (0.5 U ml 1 )

The role of salivary contents and modern technologies in the remineralization of dental enamel: a narrative review