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Dysbiosis of the gut microbiota is now recognized as a critical factor in both the initiation and progression of IBD ( 5-Aminosalicylic acid (5-ASA) remains a cornerstone of IBD therapy ( Natural polysaccharides have demonstrated considerable benefits in IBD treatment, as they reduce drug-related side effects while enhancing therapeutic efficacy ( Additionally, polysaccharide-based nanoparticles have attracted attention for their ability to improve drug stability and sustain therapeutic activity ( 2 The relationship between the intestinal microbiome and IBD Numerous studies have documented significant alterations in the intestinal microbiota of patients with IBD compared with healthy individuals (29)

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However, there was no significant change in B16 cells after different treatments