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The kidney organoids were treated with Hoechst 33342 (200 mM) for 1 h and MitoSOX Red (5 M) for 30 min at 37 C in darkness and washed with PBS, and a z-stack fluorescence image was obtained using a fluorescence microscope

10.1016/j.urology.2004.06.021 Urology

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Moreover, biochemical analyses were performed on whole brain homogenates, assessing malondialdehyde, glutathione and catalase as oxidative stress markers using standard ELISA and spectrophotometric assays

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