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We then cloned the 3-UTR fragment of VEGFA mRNA containing the putative miR-339-5p binding sites, as well as the mutant 3-UTR fragment lying upstream of the luciferase coding sequence ( Figure 4D )

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This is reflected by the activity of protective antioxidant pathways such as mitochondrial uncoupling and the Nuclear factor E2-related factor 2 (NRF2), which are weakened or bypassed in diabetes, allowing oxidative damage to accumulate [9]