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Maintaining the overall ionic strength of the CFE environment is important to mimic the cellular environment and maintain the proper function of biological processes

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The plates were incubated for 30 min at 25C before the addition of 100 l of the substrate solution (0.04 M Na 2 HPO 4 , 0.2 mm, 5,5-dithio-bis-2-nitrobenzoic acid (DTNB), 0.24 mm acethyltiocholine iodide (ATCI) in HPLC-grade water

We have shown that the glutathione pool is very insensitive to the large variations inacute amino acid input caused by daily meals