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Pellets were resuspended in lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5% (v/v) glycerol, 15 mM imidazole, 4 mM BME and 1 tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001)), with 1 ml of lysis buffer per 240 ml of overnight culture

Mitochondrial 2OG is generated by the TCA cycle or by glutaminolysis and transamination of glutamate

L.WangY.et al (2021)

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1 , 2 , 3 , 4 , 5 Dental amalgams are the most common source for elemental mercury vapor, which is a stable monoatomic gas