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Description

To understand the contribution of GCN2 to the broad rewiring of the transcriptome in Leu- or Arg-starved ES cells, we generated Eif2ak4 -deficient ES cells by CRISPR/Cas9 targeting (Fig S1B)

Because electrons like to be in pairs, free radicals seek stability by interacting withand often damagingnearby cells, proteins, or DNA

Western blotting Proteins were extracted by using radio immunoprecipitation assay (RIPA) lysis buffer (Beyotime, China) and then quantified using a BCA Protein Quantification Kit (#E112-01, Vazyme, China)

Cell viability was calculated using the formula: Antioxidant Efficacy Peripheral blood mononuclear cells (PBMCs) were isolated from 10 mL of peripheral blood obtained from healthy volunteers after ethical approval and written informed consent (PUIECHR/PIMSR/00/081734/7212)

Acta BBA Summary Keywords lysine methylation, reader domains, functional proteomics, non-histone proteins, lysine-orientated peptide libraries Citation Kupai A, Vaughan RM, Dickson BM and Rothbart SB (2020) A Degenerate Peptide Library Approach to Reveal Sequence Determinants of Methyllysine-Driven Protein Interactions