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The protein sample (250 g protein /30 L) was mixed with 270 L of UA (100 mM TrisHCl (pH 8.5) containing 8 M Urea) in the filter unit, and centrifuged at 14,000 g for 15 min

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Early diagnosis is vital as severe cases of hyperthyroidism can progress into a potentially life-threatening condition called thyroid storm, characterized by high fever, rapid heartbeat, severe agitation, vomiting, diarrhea, and dangerously low blood pressure

Age-related loss of Notch3 underlies brain vascular contractility deficiencies, glymphatic dysfunction, and neurodegeneration in mice