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The intensity and duration of the temperature increase determine the degree of temperature-induced cellular damage, with different temperatures having different effects on tumor cells [19]

For live cell-based coupled assays, the assay system needs to be separated from the intracellular space that contain high concentration of NAD(P)H, so the probe should be retained in extracellular media throughout the assay

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Various types have been developed, including synthetic enzyme mimics based on cyclodextrins, crown ethers, linear or cyclic peptides, micelles, and molecularly imprinted polymers 23,24

Gently rub the syndet bar to create a lather