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A transient cotransfection-replication assay demonstrated an essential role of K8 in ori-Lyt -dependent DNA replication as omission of K8 expression plasmid in the co-transfection led to lack of detectable DNA replication ( Both RTA and K8 are able to interact with core replication machinery proteins and recruit these proteins to ori-Lyt DNA to form a pre-replication complex (Wang et al., 2006)

Therefore, the mere presence of a chirality center may not always be sufficient to allow for the isolation or resolution of enantiomers

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Quantitative single cell determination of ERK phosphorylation and regulation in relapsed and refractory primary acute myeloid leukemia