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On day 4, the protein expression of key DE markers, SOX17 and forkhead box protein A2 (FOXA2), were evaluated by immunocytochemistry staining on 4% paraformaldehyde (PFA)-fixed cells using SOX17 mouse anti-human monoclonal antibody (Abcam) and FOXA2 rabbit anti-human monoclonal antibody (Abcam), as described below

for example, (i) particle size and surface-area-to-volume ratio, where reducing the sphere size increases the surface area per unit volume, resulting in higher drug loading efficiency (De et al., 2022)

doi: 10.3389/fphar.2021.718147 Received 09 June 2021 Accepted 17 November 2021 Published 13 December 2021 Volume 12 - 2021 Edited by Fan Jiang, Shandong University, China Reviewed by Dechang Chen, Shanghai Jiao Tong University, China Ying Li, Xian Jiaotong University, China Updates Copyright 2021 Tepes, Gojkovic, Krezic, Zizek, Vranes, Madzar, Santak, Batelja, Milavic, Sikiric, Kocman, Simonji, Samara, Knezevic, Barisic, Lovric, Strbe, Kokot, Sjekavica, Kolak, Skrtic, Seiwerth, Boban Blagaic and Sikiric