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In this method, the N -Fmoc group is deblocked using piperidine (20% v/v) in DMF at room temperature and the C -terminal residue is in an N -Boc-protected form, eg Boc-His (Trt) -OH or Boc-Tyr(tBu)-OH or equivalent

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Some prefer injecting near the injury site for localized effects, while others use subcutaneous abdominal injections for systemic distribution

References Collins, N