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10.1093/omcr/omw006 104

Revised guidelines for the clinical management of Lynch syndrome (HNPCC): Recommendations by a group of European experts

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Meanwhile, a culture of EcN pSIM18 was grown to mid-log phase at 30 C in LB + 200 g/mL hygromycin, then expression of the lambda Red proteins was induced by incubating the cells at 42 C for 15 min, followed by 15 min on ice