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The morphological features of ferroptosis are quite specific, including a reduction (disappearance) of the mitochondrial cristae, rupture of the outer mitochondrial membrane, wrinkling, darkening of the mitochondria, iron-dependent nuclei without rupture, and cell membrane rupture

demonstrated that D + Q was unable to eliminate senescent hepatocellular carcinoma (HCC) cells that had entered a state of senescence following prior doxorubicin treatment [116]

The samples were prepared and analysed by LC-MS at Duke University as previously described 55

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