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The samples were incubated for 1 h at RT, washed three times with TBST, reacted with Supersignal West Pico solution (Pierce) for 5 min, and exposed to X-ray film (Agfa)

As occurs with GSH when used against HIV, the thiol (-SH) moiety is capable of post translational protein modifications that may disable thermodynamically less stable viral proteins synthesized during infections

The pathophysiological process of CVD is multifactorial and can be affected by tea components in several processes: anti-hypertension, lipid-lowering effects, anti-oxidation, anti-inflammation, anti-proliferation, anti-angiogenesis, anti-AS, recovery of endothelial function, anti-thrombosis, myocardial protective effect (Figure 2)

Purified laccases were stored at 4 C for further analysis

Kolb H, Kempf K, Rohling M, Lenzen-Schulte M, Schloot NC, Martin S