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MichaelisMenten kinetics were calculated by first obtaining the concentration of TNB formed under the reactions ( c = A / bE ), where c is the concentration, A is absorbance, b is the path length (1 cm) and E is the molar absorptivity (molar extinction coefficient of TNB is 14,150 M 1 cm 1 (ref

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In malignancies, the subtle balance of internal redox environment between free radicals produced or quenched by cellular antioxidants and enzyme systems is grossly dysregulated