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Mechanisms of obesity and related pathologies: androgen deficiency and endothelial dysfunction may be the link between obesity and erectile dysfunction

doi: 10.1021/tx300295n

10.1210/jc.2015-2222 16

The present study examined the morphological changes of KGN cells in the aforementioned groups using electron microscopy, measured the levels of oxidative stress [2,7-dichloro-dihydrofluorescein diacetate (DCFH-DA) assay of ROS, spectrophotometry of superoxide dismutase levels, ELISA of glutathione levels, spectrophotometry of malondialdehyde (MDA) levels], and examined cell apoptosis using flow cytometry and FSHR expression using western blotting to explore the effects of LC on the response of KGN cells to oxidative stress