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McDermotts investigative team also demonstrated that most people with PAD do not have classical symptoms of intermittent claudication and that even people with asymptomatic PAD have greater functional impairment and faster functional decline than people without PAD

About 50 l of quenched sample were passed through a home-packed pepsin column using agarose immobilised pepsin (Thermo Fisher Scientific) at 40 l min 1 (20 C) and a VanGuard Pre-column Acquity UPLC BEH C18 (1.7 m, 2.1 mm 5 mm, Waters Ltd., Wilmslow, Manchester, UK) for 3 min in 0.3% formic acid in water

Bella et al

ACC converted acetyl-CoA to malonyl-CoA, which served as a physiological inhibitor of CPT1A, the decisive rate-limiting enzyme in FAO

J Pediatr 1995;126:287-92