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Protein were then eluted by adding 20 l of IP lysis buffer (with protease inhibitors and phosphorylase inhibitors added) and 5 l 5SDS loading buffer, followed by 95 C for 5 min

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A significant number of proteins involved in signaling have critical thiols (e.g., cell receptors, ubiquitinylation proteins, protein kinases, and some transcription factors)

These findings underscore the importance of baseline oxidative status, timing, and dose in determining the outcome of combined HBOT and NAC therapy