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Mercer et al., 2018)

Studies on ASD people showed that TNF cascades produce proinflammatory cytokines, leading to peripheral inflammation and activation of microglia in the brain, which indicates neuroinflammation

The following day, the coated plates were washed three times with the washing buffer in a HydroFlex microplate washer (TECAN, Mannedorf, Switzerland) and blocked with blocking buffer at 37 C for 2 h