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Female reproductive aging and oxidative stress: mesenchymal stem cell conditioned medium as a promising antioxidant

Post-treatment, cells were collected and washed 3x with ice-cold PBS, and lysed in 250 l of lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1% Triton-X-100, 1 mM EDTA, 5 mM MgCl2, 5% glycerol) freshly supplemented with the 100X Halt Protease Inhibitor Cocktail for 20 min on ice

however, because of the special identification method, not all samples were screened for hCG (Table 2)

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