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Insoluble material was removed by centrifugation at 16,000 x g and His 10 -mRuby2-GDAP1 fusion protein captured using nickel affinity chromatography followed by digestion with TEV protease overnight to liberate GDAP1 protein from the His10-Ruby tag

Elsevier health sciences

5 m thick sections were dehydrated, deparaffinized, rehydrated

First, transcriptional changes in Bmal1 and Hif-1 may not reflect direct regulation due to confounding factors

Experiments were designed to differentiate the mechanisms and subtype of kinin receptors mediating the changes in intracellular Ca2+ concentration ([Ca2+]i) induced by bradykinin (BK) in canine cultured tracheal epithelial cells (TECs)