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In vitro succinylation assay Purified GCLC proteins were incubated with different concentrations of succinyl-CoA (S1129, Sigma-Aldrich) in TBS buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl) at 37 C for 4 h

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502 Approximately 10% of ALS cases are familial ALS, with various disease-causing genes identified, including superoxide dismutase 1 (SOD1), chromosome 9 open reading frame 72 (C9ORF72), and fused in sarcoma (FUS)

In addition to reducing oxidative stress and regulating inflammation, glutathiones work as an antioxidant supports T cells, natural killer (NK) cells, and other white blood cells

Guyatt GH, Oxman AD, Vist G, et al